Polymerase chain reaction for detection of adenoviruses in stool samples

Real-time polymerase chain reaction for detection of Isospora belli in stool samples.

A real-time polymerase chain reaction (PCR) assay targeting the internal transcribed spacer 2 region of the ribosomal RNA gene was developed for the detection of Isospora belli DNA in fecal samples, including an internal control to detect inhibition during the amplification process. The assay was performed on species-specific DNA controls (n = 27) and a range of positive (n = 21) and negative (...

Development of Multiplex Reverse Transcription-Polymerase Chain Reaction for Simultaneous Detection of Influenza A, B and Adenoviruses

Background and objective:Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. ...

Detection of Ureaplasma Urealyticum in Clinical Samples from Infertile Women by Polymerase Chain Reaction

Genital Ureaplasma urealyticum infection is considered to be a sexually transmitted infection. The bacterium has been found to be involved in PID, chorioamnionitis, urethritis, respiratory distress syndrome and pneumonia in newborn, abortion and infertility. U. urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. The purp...

Detection of Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction

detection of adenoviruses from clinical samples in bone marrow transplant patients by nested pcr (polymerase chain reaction)

adenoviruses are recognized as common human pathogens that are responsible for a wide variety of infectious syndromes. bone marrow transplant patients are prone to life threatening opportunistic infections like adenoviruses. the nested polymerase chain reaction has provided an alternative, sensitive diagnostic method for detection of adenoviruses. in this study we developed pcr from hexon genes...